![]() ![]() The resulting data can be analyzed with the free Cell Ranger and Loupe Cell Browser software. Up to sixteen samples can be processed per batch within minutes. ![]() The single-cell encapsulating process is significantly faster compared to other technologies. In contrast to other protocols, the 10X controller can load “all” droplets with micro-beads, enabling single-Poisson distribution loading and thus high capture efficiencies (in contrast to double-Poisson loading of other protocols). (These numbers can be further increased with sample multiplexing.) Each bead is loaded with adapters containing one of 750,000 different barcodes for the single cell RNA-seq library preps. The 10X Genomics Single Cell suite enables high capture efficiency (of up to 65% of cells loaded) with a flexible workflow, encapsulating 500 to 20,000 cells or nuclei per library together with micro-beads into nano-droplets. Due to ongoing COVID19 testing, we have suspended customer instrument use temporarily. This is advantageous if your group is planning a large number of single-cell experiments. UC Davis users with BSL1 samples can access our 10X Chromium controller as part of our shared instruments for customer use. We can also connect you with your local 10X sales specialists and scientists for office hour appointments! We offer free joint consultations with the Bioinformatics core.
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